Metamizole or Novalgin® is a widely used well tolerated analgesic drug which is however compromised by agranulocytosis as adverse effect. Subsequent to nonenzymatic hydrolysis, the primary metabolic step is N-demethylation of 4-methylaminoantipyrine (4-MAA) to 4-aminoantipyrine (4-AA). The aim of the present study was to identify the human cytochrome P-450 enzyme (CYP) mediating this reaction. This study identified the relevant CYP using virus expressed isolated human CYP, human liver microsomes and rat liver microsomes with chemical inhibition studies. The substrate of 4-methylaminantipyrine was employed at six different concentrations (25, 50, 100, 400, 800 and 1200 µmol per l) with varying concentrations of selective inhibitors of CYP1A2 (furafylline, fluvoxamine), CYP3A4 (ketoconazole), CYP2A6 (coumarin), CYP2D6 (quinidine), CYP2C19 (omeprazole, fluvoxamine, tranylcypromine), CYP2C9 (sulphaphenazole) and CYP1A1 (alpha-naphthoflavone). 4-MAA and 4-AA were analyzed by HPLC and enzyme kinetic parameters (Km and Vmax) were determined by regression (Sigma plot 9.0). The N-demethylation of 4-MAA by microsomes prepared from baculovirus expressing human CYP was pronounced with CYP2C19. Intrinsic clearance of the most active enzymes were 0.092, 0.027 and 0.026 for the CYP enzymes 2C19, 2D6 and 1A2, respectively. Metabolism by human liver microsomes was strongly inhibited by fluvoxamin, omeprazole and tranylcypromine (IC50 of 0.07, 0.07 and 0.18, respectively) but with coumarin, sulphaphenazole, ketoconazole, moclobemid, quinidine alpha-naphthoflavone and furafylline were 0.79, 1.20, 1.36, 1.44, 3.46, 4.68 and 8.41, respectively. The enzyme CYP2C19 apparently has an important role in N-demethylation of 4-methylaminoantipyrine which should be further analyzed in clinical studies and which may also be interesting concerning the agranulocytosis.

Mediterranean journal of pharmacy and pharmaceutical sciences

In recent years, the educational landscape has changed drastically with increased connectivity and technology that promotes outside-the-box thinking and innovation. Classrooms at all levels are evolving to meet this "new normal" through virtual lessons, smart technology in schools and online access for students and parents an individual possession of knowledge, skills and experience through education certainly transforms the nature’s endowed resources as marketable products/services with ‘economic value’ is termed as human capital and knowledge economy. Enhance and enrich of human capital fillips nation’s sound and health economy is the rationale of education. Education thrives to (a) educate, enlighten and encourage teaching, (b) input of understanding and (c) spirit and urge true facts. Education policy needs a clear-cut and transparency to lead in commercialisation and determination of values and proactive to the market logic. The great challenges accompany have been thoroughly exposed in recent periods through Covid-19 crisis. Interaction and integration with the economy of the rest of Worlds Counties, has now become a new emerging facet to bring out a unifying roof of the whole-teaching contents and methodology to attain the socio-economic development. To become a world-class academic institute heavily depends upon the committed faculty, quality infrastructures, supportive administration, and learning environment. Last but not least is the hardworking and brilliance or vividness is the foremost factor of promising among the young students. The corporate philanthropy to higher education is the call of the day to ensure trueness of education system for urging the prime goal of socio-economic development. This study focuses on the emerging trends in Indian higher education.

Dr gedam kamalakar

Background: This study was conducted to determine the level of the public knowledge, attitudes and practices related to common eye diseases in the southern Indian population. Methods: A cross sectional population-based survey used a semi structured questionnaire on awareness, knowledge, attitude and practices related to eye diseases. Stratified multistage cluster random sampling method was used with a sample size of 867 adults >16 years based on, estimated awareness of cataract 70%, assuming an expected rate of 85%, design effect of 1.5, margin of error 4% with 95% confidence interval with 25% from urban areas and 75% from rural areas. A pilot study was conducted to validate the questions used in the main study. SPSS (version 19) used for analysis. Results: A total of 782/867 (90.1%) subjects participated in the survey, with females 47.4%. Awareness of major eye diseases for cataract 81.5%, glaucoma 47.8%, refractive error 74.3%, squint 89.0%, diabetic retinopathy 65.0% and age-related macular degeneration 36.7% respectively. 41.8% subjects reported wearing glasses either for distance, near or for both. Of the 777 subjects only 294 people underwent an eye examination by an ophthalmologist in the last two years (37.8%). 57.5% subjects had knowledge of the nearest eye care facility within five kilometers of their residence. Electronic media were the major source of information on eye health. Conclusions: Though the awareness levels of common eye diseases were quite high, the knowledge of cataract (10.4%) was poor. There is a need to increase the level of awareness for eye diseases like glaucoma and age-related macular degeneration.

Srinivasa reddy pallerla

Type 2 Diabetes mellitus contributes to the development or progression of many chronic and age-related pathological processes. One of the major risk factors for morbidity and mortality among patients with diabetes is renal and vascular disease as well as heart disease. Type 2 diabetes mellitus was associated with a significantly increased age of kidney disease with higher urea and creatinine levels. 76 Libyan subjects participated in the study. The subjects were divided into three categories. One group hosts diabetes case (n=40) while the other group serves as a control (n=12) and the other group has no type 2 diabetes mellitus but urea and creatinine levels are elevated (n=36). After oral informed consent of the study participants, 5 ml of venous blood was drawn under sterile conditions for whole blood analysis. Fasting blood sugar was analyzed. Urea and creatinine levels were compared between diabetic and non-diabetic patients. However, a statistical analysis was performed using IBM SPSS version 26 where the correlation between parameters was analyzed by an independent samples t-test. In this study, patients with type 2 diabetes mellitus caused a significant increase in urea and creatinine compared to the control group. The study indicated that type 2 diabetes mellitus increases the damage to the kidney function.

Mediterranean journal of pharmacy and pharmaceutical sciences

Provision of clean water is one of the United Nations Sustainable Development Goals. Water quality assessment as an ongoing exercise, in view of the threat of contamination from natural sources and human activities, plays a pivotal role in the sustainable management of water resources. Potable water samples were collected from Enugu Metropolis for water quality assessment. The physicochemical parameters were determined using APHA (1995 - 1998) protocols, and the presence of 7 heavy metals (Chromium, Zinc, Manganese, Silver, Cadmium, Iron and Lead) in the waters were determined using Atomic Absorption Spectrophotometer (AAS). The results of the physicochemical analysis of the four samples show a pH range of 8.60 - 8.95mg/l; this result showed that pH of the water samples were slightly alkaline and above the WHO stipulated range of 6.50 - 8.50. Acidity ranged from 5.00 - 6.88mg/l, while alkalinity values were between 7.52 - 13.00mg/l. The total dissolved solid (TDS) and total solids (TS) was present in very minute quantities and below their permissible limit of 0.45mg/l; while total suspended solid (TSS) was not present at all. The heavy metal analysis showed that all but 2 heavy metals (Cadmium and Lead) were below the permissible standard set by the WHO in all four samples. Cadmium was present in samples A and C with values 0.021mg/l and 0.006mg/l respectively, which is above its WHO limit of 0.005mg/l; while Lead was present in samples A, B and C with values of 0.021mg/l, 0.011mg/l and 0.015 mg/l respectively, which were above the WHO limit of 0.010mg/l. Given the well-documented toxicity of certain metals, there is a need for safety checks to be carried out in potable water companies to address the source of this contamination and ensure production of safer drinking water.

Okechukwu obed chukwuemeka

If you already have a research project in mind, the research planning phase must be completed with the formulation of a wellstructured research proposal that must be submitted to an ethics committee for approval before your project can start. Generally, a research proposal contains the following elements: Title, abstract, introduction, objectives, methodology, timeline and milestones, ethical considerations, budget, appendices, and references. Whether you are a medical student or a researcher, knowing how to write a research proposal is an important skill to start any research project. This guide will give you the skills to write a successful research proposal.

Karishma

Analgesic activity on two Cleome scaposa and Cleome brachycarpa by applying pressure-induced pain model

Dr. salman ahmed

Iatrogenic splenic infarction is a rare clinical entity that can result from various abdominal surgical and invasive procedures. We report a case of iatrogenic splenic infarction in a 29-year-old female who presented with hemoptysis and was found to have pulmonary sequestration. During the embolization of the anomalous artery supplying the pulmonary sequestration, she developed severe pain and was found to have a splenic infarction, caused by a part of the coil that dislodged during the procedure. The patient showed good response to conservative management and was discharged in good condition.

Karishma

Background: The first wave of the COVID-19 pandemic was associated with significant morbidity and mortality among healthcare workers worldwide. The present study aimed to assess the knowledge, attitudes, and practices of healthcare workers toward COVID-19 at Ataq General Hospital, and three other hospitals and health centers in Shabwah Governorate, Yemen. Materials and Methods: From January 1, 2022, to February 28, 2022, a cross-sectional survey of healthcare workers was conducted in the city of Ataq, Shabwah Governorate at the following hospitals: Ataq General Hospital, Al Shefa’a Hospital, Al Aafiah Hospital, and COVID-19 Isolation Center. Results: A total of 107 healthcare workers completed the survey. Their mean age was 28.17 ± 7.73 years, 79 (73.8%) of them were male and 28 (26.2%) were female. The overall knowledge was good, with a score of 19 out of 21; however, most participants were unaware of some of the extra-respiratory symptoms of the disease, such as diarrhea and confusion, and about 57% of them were unaware that eating or interacting with wild animals may contribute to the infection with the COVID-19 virus. Attitude analysis of the participants revealed that about half of those surveyed do not believe that Yemen can contain COVID-19. In general, the practice of the participants was good. Conclusion: Although the overall knowledge score in this study was good, most respondents could not recognize some of the extrapulmonary manifestations of COVID-19 and were unaware of the possibility of transmission of the disease from wild animals. In addition, about half of those surveyed do not believe that Yemen can contain COVID-19

Karishma

Hypoglycemia is a common complication of intensive diabetes mellitus therapy. This study was aimed to review the profile of admitted Libyan cases of non-pregnant adults and adolescents. A retrospective study for patients admitted to Tripoli Diabetes Hospital, Tripoli, Libya. Data were collected from medical files of diabetic patients with hypoglycemia from January 2017 to July 2018. Thus, during this period, a total of 29 cases with hypoglycemia were admitted to the hospital. Their age was ranged from 18 to 86 years in which 76.0% were female, 25.0% were non-smokers, 59.0% were married and 55.0% were non-employers. Patients with diabetes mellitus (86%) showed hypoglycemia attack per month of 3.90 ± 2.25. From the 25 cases with diabetes mellitus, 7.0% were newly diagnosed (> one year), 45.0% their duration of diabetes mellitus were more than 9 years and 35.0% were less than nine years. Their Hemoglobin A1C was on target (6.5–7.0%) in 21.0% of the cases, in which 17.0% were on below target range (< 6> 7.0%). 14.0% were on glibenclamide, 3.0% on glimepiride and 69.0% on insulin. Patients on insulin therapy twice daily in 28.0%, triple in 17.0% and basal bolus regime in 24.0%. Hypoglycemia unawareness was presented in 17.0%, hypoglycemia was major in 45.0% of the cases, and in 38.0% were in daytime. Insulin induced hypoglycemia were in 66.0%, and suicidal attempt were in 10.0%. Exercise induced hypoglycemia 10.0% and 3.0% of cases with malignancy induced hypoglycemia. Co-exist systemic illness was mainly absent in 69.0% but cardiovascular diseases in 14.0% and renal diseases in 7.0%. Majority of cases were discharged in good condition (86.0%) and 14.0% discharged against medical advice. Hypoglycemia mostly occurs in patients with diabetes mellitus treated with insulin and in most the cases were reversible and saved with good management. Thus, patient education is a fundamental issue in prevention and reducing complications of hypoglycemia in patients with diabetes mellitus.

Mediterranean journal of pharmacy and pharmaceutical sciences

Abstract- The role of support vector machine in the evaluation of English teaching effect is very important, but there is a problem of inaccurate evaluation of results. The traditional English teaching mode cannot solve the accuracy and efficiency of the effect evaluation of students' English teaching and cannot meet the requirements of English teaching effect evaluation. Therefore, this paper proposes a neural network algorithm to innovate and optimize the analysis of support vector machines. Firstly, the relevant theories are used to construct a multi-index English teaching effect evaluation system with teachers and students as the main body, and the indicators are divided according to the data requirements of English teaching effect evaluation indicators to reduce the support vector machine in the interfering factor. Then, the neural network algorithm is used to solve the optimal solution of kernel function parameters and regularization parameters of the support vector machine, and the support vector machine scheme is formed, and the support vector machine results are carried out Comprehensive analysis. MATLAB simulation shows that the evaluation accuracy of the English teaching effect of the neural network algorithm and the support vector machine under certain evaluation criteria Optimal, short evaluation time.

Lokesh n s

The aim of the study is to: assess the effectiveness of structured teaching programme on prevention of selected postoperative complications among 300 patients undergoing abdominal surgeries in selected hospitals. A pre-experimental research design and convenient sampling technique was adopted study. Data were collected by using structured interview schedule and structured questionnaire was used to assess the existing knowledge regarding prevention of selected post operative complications. Shows that majority (42.70%) subjects had inadequate knowledge and (18.30%) had moderate knowledge in pre test. In post test majority 44 (73.3%) had adequate knowledge and 16 (26.7) had moderate knowledge. The pre test mean score was 16.15 with the standard deviation of 4.65, whereas in post test the mean score was 30.7 with the standard deviation of 5.387 which indicates that structured teaching programme is statistically significant at P<0.01 level. There was significant difference between the pre test and post test knowledge scores t=20.56, P<0.01 level. There was a significant association between pre test knowledge scores with their education and no association between age, gender, religion, occupation, marital status, type of family, place of domicile, dietary pattern, personal habits and source of health information at P<0.01 level. Author concluded that that post test knowledge scores was improved after structured teaching programme. So structured teaching programme was effective in increasing the knowledge level regarding prevention on selected post operative complications among patients undergoing abdominal surgeries.

Prof.k.c yadav

The ability of antimicrobial peptides (AMPs) for effective binding to multiple target microbes has drawn lots of attention as an alternative to antibodies for detecting whole bacteria. We investigated pathogenic Escherichia coli (E. coli) detection by applying a microfluidic based biosensing device embedded with AMP-labeled beads. According to a new channel design, our device is reusable by the repeated operation of detection and regeneration modes, and the binding rate is more enhanced due to even distribution of the bacterial suspension inside the chamber by implementing influx side channels. We observed higher binding affinity of pathogenic E. coli O157:H7 for AMP-labeled beads than nonpathogenic E. coli DH5α, and the fluorescence intensity of pathogenic E. coli was about 3.4 times higher than the nonpathogenic one. The flow rate of bacterial suspension should be applied above a certain level for stronger binding and rapid detection by attaining a saturation level of detection within a short time of less than 20 min. A possible improvement in the limit of detection in the level of 10 cells per mL for E. coli O157:H7 implies that the AMP-labeled beads have high potential for the sensitive detection of pathogenic E. coli at an appropriate flow rate.

Myung-suk chun

PHYSICO-CHEMICAL ANALYSIS OF RHIZOSPHERIC SOIL OF MAIZE (Zea mays L.) AND ITS EFFECTS ON MAIZE DISEASE INCIDENCE (IN VIVO) IN KARNATAKA

Veerabhadraswamy al

Three species of arbuscular mycorrhizal fungi (Glomus fasiculatum, Glomus mossae and Acaulispora laevis) were used as bio-agents to manage black bundle disease of maize caused by C.acremonium. The results revealed that colonization of arbuscular mycorrhizal fungi in root system of the host reduce the percentage of disease incidenceconsiderably.In the pots inoculated with G. fasiculatum no disease incidence (0.0%) was recorded whereas, in the pots inoculated with A. leavis and G. mossae 16.66 % of disease incidence was recorded and the pots treated with pathogen shows 66.66% of disease incidence compare to control. Among the three bio-agents, Glomus fasiculatumproved to be more effective in managing the disease followed by G.mossae and A. laevis. In addition, all the three AM fungi enhanced the plant growth when they are used alone as inoculum as compared to dual inoculation with the C. acremonium and overall control. This clearly suggests that, AM fungi if used, can serve dual purpose. It can be used as bio-control agent as it shows negative antagonistic interaction soil borne plant pathogens and used as growth promoter because of the ability to supply macro and micro nutrients to the host plants.

Veerabhadraswamy al

The bioactive phytochemicals are the most important bioactive compounds against insect herbivores. These phytochemicals produced by many legumes and react herbivore attacks. Therefore, keeping in mind the said facts, this review discusses the potential use of bioactive compounds like Proteinase inhibitors, trypsin inhibitors, lectins, vicillins (7S storage proteins), phenolics, amino acids, sugars and a-amylase inhibitors in legume plants as most important weapons that confer resistance against herbivorous insects. These inhibitors inhibiting proteases present in the larval gut and has insecticidal potential against insect pests. This review demonstrates the potential of natural phytochemicals from legume plants and or artificially diet incorporate to inhibit the development of pathogens and insect pests. Thus, legumes can be used to produce phytochemicals as bio-pesticides and minimize or avoid the use of agrochemicals in crop protection. This ability making them interesting bioactive for next generation of sustainable pesticides for more sustainable agriculture.

Dr. babu lal jat

This present study evaluated the quality attributes of functional bread developed from the blends of wheat and soy-okara (SOF) flour. A completely randomized design which generated six (6) experimental runs based on different combinations of wheat (60–90%) and soy-okara (10–40%) flours was adopted for the production of functional bread. The flour blends were processed into bread and analyzed for sensory properties. The optimum flour combination in obtaining higher sensory acceptability was 85.34% WHF and 14.66% SOF with desirability function of 85% using numerical optimization techniques. The control (100% whole-wheat bread) and optimized bread produced were assessed for physicochemical, antioxidant properties and sensory qualities. The carbohydrate, calcium, phosphorus, and sodium of the optimized bread were significantly higher (p<0.05) than in the control sample having 58.14 and 75.14%, 128.80, and 75.60 mg/100 g, 68.18 and 63.77 and 13.83 ppm and 12.62 ppm respectively. The total flavonoid and phenolic contents of the control and optimized bread were significantly (p<0.05) different with values ranging from 92.77 to 114.86 mg/100 g and 66.43 to 57.51 mg/100 g respectively. A significant variation was observed in the sensory qualities between the control and optimized bread. The study revealed that the nutritional and antioxidant properties of the developed functional bread validate its potential health-promoting effects.

Okechukwu obed chukwuemeka

Madrasah Ibtidaiyah KAHASRI merupakan lembaga yang ditunjuk oleh Kementerian Agama RI untuk melaksanakan Kurikulum 2013 atau yang disebut K13 baik pada pembelajaran tematik maupun pembelajaran agama sesuai dengan Keputusan Direktur Jendral Pendidikan Islam Nomor 3525 tahun 2017 tentang Penetapan Pelaksanaan Kurikulum 2013. Berdasarkan latar belakang tersebut, maka fokus penelitian adalah: 1) Bagaimana perencanaan pembelajaran kurikulum 2013 di Madrasah Ibtidaiyah KAHASRI Kota Probolinggo Tahun Pelajaran 2018/2019? 2) Bagaimana pelaksanaan pembelajaran kurikulum 2013 di Madrasah Ibtidaiyah KAHASRI Kota Probolinggo Tahun Pelajaran 2018/2019?, 3) Bagaimana evaluasi pembelajaran kurikulum 2013 di Madrasah Ibtidaiyah KAHASRI Kota Probolinggo Tahun Pelajaran 2018/2019? Hasil penelitiannya adalah: 1) Perencanaan pembelajaran K13, guru di Madrasah Ibtidaiyah KAHASRI Kota Probolinggo membuat perangkat pembelajaran berupa Kalender Pendidikan, RPE, Prota, Promes, Silabus, dan RPP. Dalam pembuatan perangkat pembelajaran ada guru yang membuat perangkat pembelajaran secara individu dan ada yang dengan bersama KKG (Kelompok Kerja Guru). (2) Pelaksanaan pembelajaran K13 di Madrasah Ibtidaiyah KAHASRI Kota Probolinggo memiliki 3 kegiatan yaitu, kegiatan pendahuluan, inti, dan penutup. Pada kegiatan pendahuluan diawali dengan memberikan apersepsi, memberikan stimulus/motivasi, menyampaikan tujuan dan manfaat pembelajaran. Pada kegiatan inti guru telah melakukan kegiatan saintifik yang meliputi kegiatan mengamati, menanya, mencoba, mengasosiasi/mengumpulkan, dan mengkomunikasikan. Pada kegiatan penutup dilakukan dengan membuat kesimpulan dari materi yang telah dipelajari, merefleksikan materi yang diajarkan, dan memberikan tindak lanjut berupa penugasan. (3) Evaluasi pembelajaran K13 di Madrasah Ibtidaiyah KAHASRI Kota Probolinggo menggunakan model penilaian autentik yang meliputi 3 aspek yaitu: aspek sikap, aspek pengetahuan, dan aspek keterampilan.

Educare: journal of primary education

India's hotel and hospitality industry occupancy decreased significantly in the first quarter of 2020, as the outbreak of COVID-19 affects different segments of the market. The outbreak of the novel coronavirus (COVID-19) has inflicted a blow on industries around the world, but maybe none as devastating as hospitality and travel. Depending on the length of the pandemic, companies across the industry adjusted growth forecasts for 2020, estimated profits to be conservatively 40-50 percent lower than expected before the outbreak. The effect on hospitality demand is greater than many other sectors, but even among hotels, air and cruise ships, and restaurants it is varied. Hotels and airlines operate at half capacity, with both business and leisure travelers canceling scheduled trips and not arranging any for the near future.The present article aims to study some novel practices for the survival of hospitality industries once the lockdown is over.

Ketan chande

The main purpose of the paper is to determine the correlation of consumers' demographic factors on the impulse buying behavior with respect to a number of single impulsivity indicators and one collective indicator. The paper consists of theoretical and research aspects. The first part encompasses theoretical insights into the secondary research regarding impulse buying while the practical part presents the methodology and primary research results. With respect to the subject matter, research goals as well as previous findings and primary research results, corresponding hypotheses were set and mainly confirmed. Inter variable correlation and regression analysis has been performed to test the hypothesis. The results showed that demographic factors, such as the disposable income and age, are related to most impulse buying indicators and to the impulsivity collective indicator. However, educational qualification and gender produced marginal association with impulsive buying behavior. The paper also summarizes research limitations as well as the work contribution and future research guidelines.

Abu bashar